Physiology Core photo

The Physiology Core Facility provides basic and biological researchers with a central resource for creating models of animal physiology and pathophysiology. While our main focus is exploring the effects of cardiovascular disease and cancer in rodents, we can provide support to any aspect of animal research. We offer both invasive and non-invasive techniques for collecting in vivo physiological data. We provide:

  • Equipment
  • Services
  • Training
  • Method development
  • Data measurement/analysis

Our staff has extensive surgical experience with a variety of animal models, strains, species and techniques. We can evaluate your needs and provide technical assistance with experimental design and technical training.

How It Works

Researchers may request our trained facility staff to perform techniques or may use the available equipment themselves once trained. Once approved, researchers can use the Booked electronic scheduling platform to remotely reserve stations or equipment.

USD and USD-affiliated researchers may use for the facility for free. External charge rates may apply for outside entities. Contact Core Manager Jessica Freeling at jessica.freeling@usd.edu for a quote or details about available services.

Highlighted Techniques

The Physiology Core Facility works with researchers to use available equipment in new ways to accomplish research goals.

Learn about our novel 3-D colon reconstruction technique.

Available Stations

Surgical Station

Surgical Station

Provides researchers with the necessary equipment to perform survival or non-survival rodent procedures.

  • Fully-equipped surgical space, including: Anesthesia system, pressure-controlled ventilator, warming pad, surgical microscope and intubation stand
  • Rodent Hemodynamics System- Powerlab System (ADInstruments) with LabChart 8 software and MPVS Ultra Pressure Volume Loop (PV Loop) System
  • Non-Invasive Blood Pressure (NIBP) System - IITC

Ultrasound Station

Ultrasound Station

Enables the collection of high-resolution microultrasound images for cardiovascular and cancer research in the rodent model.

  • Vevo 2100 Ultrasound Imaging System with VevoLAB software, including: Standard echocardiogram functions (B-mode and M-mode echocardiography); Speckle Tracking Echocardiography (STE) (strain analysis); Vascular perfusion analysis (non-linear contrast and microbubble capability); Tissue/tumor quantification (3-D reconstruction and volume assessment); Color and Pulsed Wave (PW) doppler
  • Isoflurane Anesthesia System

Multi-Use Station

Multi-Use Station

Provides several imaging modality options. Extracted whole rodent organs or tissues, such as tumors, can be imaged with high resolution. Live animals may be tagged with near-infrared fluorescent probes to target tissues of interest.

  • Isoflurane anesthesia system
  • Odyssey Classic LI-COR Infrared Imager with MousePOD in vivo Imaging Accessory
  • Leica S6D Stereomicroscope with EC3 digital camera

Necropsy Station

Necropsy Station

Provides workspace for tissue collection.

  • Secondary surgical or necropsy station
  • Isoflurane anesthesia system
  • Euthanasia equipment

Available Services/Training

  • Aseptic surgical technique
  • Injection and intubation techniques
  • Survival surgery-cardiovascular challenge model induction, general surgery
  • Acute surgery- hemodynamic recordings
  • Equipment usage
  • Data collection and analysis
  • Experimental design
  • New model or method establishment
  • Necropsy and tissue collection
  • IACUC protocol development
  • Ultrasonography

Contact Us

Jessica Freeling, M.S., V.T., LATG
Core Laboratory Manager
Lee Medicine Room 124
jessica.freeling@usd.edu
physiology.core@usd.edu
(605) 658-6310
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